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pet 28 vector  (Addgene inc)


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    Structured Review

    Addgene inc pet 28 vector
    Pet 28 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+28/pET28(a)-hFX-HA+(Plasmid+%2314991)/pm40419540-218-48-54
    Average 94 stars, based on 15 article reviews
    pet 28 vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Binding Assay:

    Article Title: PIPKIγ targets to the centrosome and restrains centriole duplication
    Article Snippet: Mouse antibodies against the following proteins were used: centrin 2 (20H5, a generous gift from Jeffrey Salisbury), ODF2 (Abnova), C-Nap1 (BD Biosciences), His (HIS.H8, Millipore), GFP (Roche); α-tubulin (DM1A), γ-tubulin (GTU-88), FLAG (M2) and HA (HA-7) were from Sigma; Myc (9E10), HsSAS-6 (91.390.21) and cyclin B (GNS1) were from Santa Cruz. .. Anti-ninein (Y-16) antibody was from Santa Cruz and maltose binding protein antibody (7G4) was from Sigma. cDNAs encoding full-length or truncated human PIPKIγ ( Ling et al., 2002 ), PLK4 and CEP152 were obtained by PCR and subcloned into pCMV–HA, pcDNA3–FLAG, pGEX–4T-1, pET-28, pET-42 or pMAL–C2X constructs. pcDNA3-Myc–PLK4 was obtained from Addgene. .. HA–PIPKIγ kinase dead (KD) and RNAi-resistant HA–PIPKIγ-WT or –KD, which contain mismatches to the HsPIPKIγ-O1 siRNA, were generated using QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Polymerase Chain Reaction:

    Article Title: PIPKIγ targets to the centrosome and restrains centriole duplication
    Article Snippet: Mouse antibodies against the following proteins were used: centrin 2 (20H5, a generous gift from Jeffrey Salisbury), ODF2 (Abnova), C-Nap1 (BD Biosciences), His (HIS.H8, Millipore), GFP (Roche); α-tubulin (DM1A), γ-tubulin (GTU-88), FLAG (M2) and HA (HA-7) were from Sigma; Myc (9E10), HsSAS-6 (91.390.21) and cyclin B (GNS1) were from Santa Cruz. .. Anti-ninein (Y-16) antibody was from Santa Cruz and maltose binding protein antibody (7G4) was from Sigma. cDNAs encoding full-length or truncated human PIPKIγ ( Ling et al., 2002 ), PLK4 and CEP152 were obtained by PCR and subcloned into pCMV–HA, pcDNA3–FLAG, pGEX–4T-1, pET-28, pET-42 or pMAL–C2X constructs. pcDNA3-Myc–PLK4 was obtained from Addgene. .. HA–PIPKIγ kinase dead (KD) and RNAi-resistant HA–PIPKIγ-WT or –KD, which contain mismatches to the HsPIPKIγ-O1 siRNA, were generated using QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Construct:

    Article Title: PIPKIγ targets to the centrosome and restrains centriole duplication
    Article Snippet: Mouse antibodies against the following proteins were used: centrin 2 (20H5, a generous gift from Jeffrey Salisbury), ODF2 (Abnova), C-Nap1 (BD Biosciences), His (HIS.H8, Millipore), GFP (Roche); α-tubulin (DM1A), γ-tubulin (GTU-88), FLAG (M2) and HA (HA-7) were from Sigma; Myc (9E10), HsSAS-6 (91.390.21) and cyclin B (GNS1) were from Santa Cruz. .. Anti-ninein (Y-16) antibody was from Santa Cruz and maltose binding protein antibody (7G4) was from Sigma. cDNAs encoding full-length or truncated human PIPKIγ ( Ling et al., 2002 ), PLK4 and CEP152 were obtained by PCR and subcloned into pCMV–HA, pcDNA3–FLAG, pGEX–4T-1, pET-28, pET-42 or pMAL–C2X constructs. pcDNA3-Myc–PLK4 was obtained from Addgene. .. HA–PIPKIγ kinase dead (KD) and RNAi-resistant HA–PIPKIγ-WT or –KD, which contain mismatches to the HsPIPKIγ-O1 siRNA, were generated using QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).



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